Science and Technology news... :: View topic

Nina · Guest

Hi,

I am very ignorant of the field of image processing, so i apologize if
my question is not worded in correct terms or. worse, has an obvious
answer!

I have taken some IF and FISH images of fission yeast nucleus which is
much smaller compared to the mammalian nucleus; so i really need to
zoom in to see my signals more clearly. But as you know, when you
zoom in, the image gets very "pixeled" (sorry, i do not know what the
term is). So I was wondering if there is a process that I can have my
images go through after zooming in to make them look more clear and
less 'pixeled looking'.

I would much appreciate if any one could point me to the right
direction.

Thank you,

Nina

Guest · Posted: Tue Oct 28, 2008 6:08 pm Post subject: Re: Zoom in and 'pixeled' images

Hi Nina,

You can avoid the pixelated (or blocky) appearance if instead of
replicating pixels you interpolate. How to do it depends on the
software you are using. Matlab has linear and cubic 2D and 3D
interpolation and, if I remember correctly, Photoshop and ImageJ also
offer zooming by interpolation. However, you should be aware that
zooming (by whatever method) does not give you more detail than is
already available in the image. Zooming in a blurry image gives you a
larger but equally blurred image. Immunofluorescence and FISH images
are typically limited by the diffraction of light at the objective and
possibly by scattering throughout the sample.

HTH,

Jomar

On Oct 27, 8:23 pm, Nina <hinin...@gmail.com> wrote:
[quote]Hi,

I am very ignorant of the field of image processing, so i apologize if
my question is not worded in correct terms or. worse, has an obvious
answer!

I have taken some IF and FISH images of fission yeast nucleus which is
much smaller compared to the mammalian nucleus; so i really need to
zoom in to see my signals more clearly. But as you know, when you
zoom in, the image gets very "pixeled" (sorry, i do not know what the
term is). So I was wondering if there is a process that I can have my
images go through after zooming in to make them look more clear and
less 'pixeled looking'.

I would much appreciate if any one could point me to the right
direction.

Thank you,

Nina[/quote]

4N · Guest

[quote]I have taken some IF and FISH images of fission yeast nucleus which is
much smaller compared to the mammalian nucleus; so i really need to
zoom in to see my signals more clearly. But as you know, when you
zoom in, the image gets very "pixeled" (sorry, i do not know what the
term is). So I was wondering if there is a process that I can have my
images go through after zooming in to make them look more clear and
less 'pixeled looking'.
[/quote]
try changing the interpolation function using something like Lanczos that
has a good tradeoff between sharpening and jagged edges, edge directed
resize could help too (you may look for the application called SAR for this)
since it produces smooth and clear edges.
Also you can apply Unsharp mask or you can change the gamma to increase the
contrast in your images.
I hope this helps.

Nina · Guest

Thank you very much for both comments! I will try to figure out how
to actually do what you have suggested!

Nina

jrg.sanchez · Guest

On Oct 27, 10:23 pm, Nina <hinin...@gmail.com> wrote:
[quote]Hi,

I am very ignorant of the field of image processing, so i apologize if
my question is not worded in correct terms or. worse, has an obvious
answer!

I have taken some IF and FISH images of fission yeast nucleus which is
much smaller compared to the mammalian nucleus; so i really need to
zoom in to see my signals more clearly. But as you know, when you
zoom in, the image gets very "pixeled" (sorry, i do not know what the
term is). So I was wondering if there is a process that I can have my
images go through after zooming in to make them look more clear and
less 'pixeled looking'.

I would much appreciate if any one could point me to the right
direction.

Thank you,

Nina
[/quote]
there are some filters that allows to enlarge your images preserving
edges (search for "edge preserving filtering" in google). Take a look
to the "bilateral filter" and "anisotropic diffusion smoothing". I
think that Photoshop has something of this.

Jorge

4N · Guest

"Nina" <hinina99@gmail.com> ha scritto nel messaggio
news:3d65402b-52fe-4386-bf36-d701ea964a6e@u75g2000hsf.googlegroups.com...
[quote]Thank you very much for both comments! I will try to figure out how
to actually do what you have suggested!
[/quote]
use an image editor like Adobe Photoshop or (for free) gimp or paint.net

Przemys?aw Skurowski · Guest

Nina pisze:
[quote]Hi,

I am very ignorant of the field of image processing, so i apologize if
my question is not worded in correct terms or. worse, has an obvious
answer!

I have taken some IF and FISH images of fission yeast nucleus which is
much smaller compared to the mammalian nucleus; so i really need to
zoom in to see my signals more clearly. But as you know, when you
zoom in, the image gets very "pixeled" (sorry, i do not know what the
term is). So I was wondering if there is a process that I can have my
images go through after zooming in to make them look more clear and
less 'pixeled looking'.

I would much appreciate if any one could point me to the right
direction.

Thank you,

Nina
[/quote]
if you could repeat these experiments than i suggest you to perform
series of images that you could use to generate so called
superresolution images then you could get more details in image than
your camera is able to acquire.

Andrew_M · Guest

[quote]I have taken some IF and FISH images of fission yeast nucleus which is
much smaller compared to the mammalian nucleus; so i really need to
zoom in to see my signals more clearly. But as you know, when you
zoom in, the image gets very "pixeled" (sorry, i do not know what the
term is). So I was wondering if there is a process that I can have my
images go through after zooming in to make them look more clear and
less 'pixeled looking'.
[/quote]
You may try my prog, availible at http://www.smartfills.com/Html/PhotoToPicture.zip
.. This one might be useful for science applications, because it
produces something like sketch. One may change some input parameters
and get output, more or less like its prototype. Enlargement up to
800% with preserved sharpness is availible too.


www.GetXFactor.com Leading Technology, Science, Agriculture News and information	Part of the Identityscape.com network... getxfactor.com jmoodmusic.com smartbusinesschoices.com mintdepot.com lowfaresalways.com evangelicalview.com shoppingpodder.com soproudlywehail.com webnews.ws currenthumor.com

Science and Technology news... Forum Index -> Image Processing Forum
Page 1 of 1