j_thomas
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 Posted: Fri Oct 10, 2008 4:57 pm Post subject: Green Fluorescent Protein(GFP)[ Nobel Prize 2008] |
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Green florescent protein (GFP) has revolutionized research in medicine
and biology, enabling scientist to get a visual fix on how organs
function on the spread of disease and the response of infected cells
to treatment,GFP has a typical beta barrel structure, consisting of
one =E2-sheet with a alpha helix containing the fluorophore running
through the center, while the tightly packed barrel shell protects the
flurophore from quenching by the surrounding microenviornment,the
inward facing side chains of the barrel induce specific cyclization
reactions on the tripeptide Ser65-Tyr66-Gly67 that lead to fluorophore
formation. This occurs in a series of discrete steps with distinct
excitation and emission properties.
In 2008 Nobel Chemsitry prize was announced to Osamu Shimomura of
Japan and American duo Martin chalfie and Roger Tsien for deriving
Florescent protein from a jellyfish Aequorea Victoria.
http://bioisolutions.blogspot.com/2008/10/green-fluorescent-proteingfp.html |
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DK
Guest
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| Posted: Sat Oct 11, 2008 9:46 pm Post subject: Re: Green Fluorescent Protein(GFP)[ Nobel Prize 2008] |
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In article <gco1gu$1bcf$1@darwin.ediacara.org>, j_thomas <julianthomasp@gmail.com> wrote:
[quote]Green florescent protein (GFP) has revolutionized research in medicine
and biology, enabling scientist to get a visual fix on how organs
function on the spread of disease and the response of infected cells
to treatment,GFP has a typical beta barrel structure, consisting of
one =E2-sheet with a alpha helix containing the fluorophore running
through the center, while the tightly packed barrel shell protects the
flurophore from quenching by the surrounding microenviornment,the
inward facing side chains of the barrel induce specific cyclization
reactions on the tripeptide Ser65-Tyr66-Gly67 that lead to fluorophore
formation. This occurs in a series of discrete steps with distinct
excitation and emission properties.
In 2008 Nobel Chemsitry prize was announced to Osamu Shimomura of
Japan and American duo Martin chalfie and Roger Tsien for deriving
Florescent protein from a jellyfish Aequorea Victoria.
[/quote]
Right. So the guy who who first realized the potential of GFP fusions,
did the cloning and sequencing, then lost funding (because NIH peer
review repeatedly pointed to how irrelevant to health and generally
not useful this research is), was denied tenure and forced out of
academic science DID NOT get the prize. Instead, the already tenured
guys recieved his clone as he was closing the lab. They had the
money to push forward and demonstrate usefullness of the approach
and eventually got the Nobel.
The guy who had the idea and was there first is driving shuttle bus
to make ends meet these days. Very inspiring story!
http://www.npr.org/templates/story/story.php?storyId=95545761
http://www.conncoll.edu/ccacad/zimmer/GFP-ww/prasher.html
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