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 Posted: Tue Oct 07, 2008 9:24 pm Post subject: Life span prediction from the rate of age-related DNA demeth |
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Exp Gerontol. 1995 Sep-Oct;30(5):475-84. Links
Life span prediction from the rate of age-related DNA demethylation in
normal and cancer cell lines.Mazin AL.
Laboratory of Molecular Bases of Ontogenesis, A.N. Belozersky
Institute of Physico-Chemical Biology, M.V. Lomonosov State
University, Moscow.
A method has been proposed for the Hayflick Limit prediction by the
analysis of the 5-methylcytosine content in DNA at earlier and later
cell passages. The following facts were used as the basis of the
method: (i) the rate of m5C loss from DNA remains approximately
constant during cell divisions and it does not depend on the cell
donor age; (ii) this rate is inversely proportional to the Hayflick
Limit as well as to the life span of cell donor species; (iii) the
period corresponded to loss of all m5C residues from the genome
coincides with or somewhat exceeds the Hayflick Limit of normal cells.
The prognosis of the Hayflick Limit has usually been found in good
agreement with the experimental evidences for various human, hamster,
and mouse cell lines. The method proposed may be used for early
detection of precrisis and cancer cells. The age-related m5C loss may
result from accumulation of the m5C-->T+C transitions occurring with
DNA methylation in every cell division.
PMID: 8557095 [PubMed - indexed for MEDLINE] |
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