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 Posted: Fri Jul 25, 2008 8:15 pm Post subject: Forty percent and eighty percent methionine restriction decr |
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Biogerontology. 2008 Jun;9(3):183-96. Epub 2008 Feb 19. Links
Forty percent and eighty percent methionine restriction decrease
mitochondrial ROS generation and oxidative stress in rat liver.Caro P,
Gómez J, López-Torres M, Sánchez I, Naudí A, Jove M, Pamplona R, Barja
G.
Departamento de Fisiología Animal-II, Facultad de Ciencias Biológicas,
Complutense University, c/Jose Antonio Novais-2, Madrid 28040, Spain.
Dietary restriction (DR) lowers mitochondrial reactive oxygen species
(ROS) generation and oxidative damage and increases maximum longevity
in rodents. Protein restriction (PR) or methionine restriction (MetR),
but not lipid or carbohydrate restriction, also cause those kinds of
changes. However, previous experiments of MetR were performed only at
80% MetR, and substituting dietary methionine with glutamate in the
diet. In order to clarify if MetR can be responsible for the lowered
ROS production and oxidative stress induced by standard (40%) DR,
Wistar rats were subjected to 40% or 80% MetR without changing other
dietary components. It was found that both 40% and 80% MetR decrease
mitochondrial ROS generation and percent free radical leak in rat
liver mitochondria, similarly to what has been previously observed in
40% PR and 40% DR. The concentration of complexes I and III, apoptosis
inducing factor, oxidative damage to mitochondrial DNA, five different
markers of protein oxidation, glycoxidation or lipoxidation and fatty
acid unsaturation were also lowered. The results show that 40%
isocaloric MetR is enough to decrease ROS production and oxidative
stress in rat liver. This suggests that the lowered intake of
methionine is responsible for the decrease in oxidative stress
observed in DR.
PMID: 18283555 [PubMed - in process] |
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Taka
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| Posted: Sat Jul 26, 2008 6:31 am Post subject: Re: Forty percent and eighty percent methionine restriction |
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Good work Barja you are doing over there BUT -
On Jul 26, 5:15 am, timoth...@my-deja.com wrote:
[quote]Biogerontology. 2008 Jun;9(3):183-96. Epub 2008 Feb 19. Links
Forty percent and eighty percent methionine restriction decrease
mitochondrial ROS generation and oxidative stress in rat liver.Caro P,
Gómez J, López-Torres M, Sánchez I, Naudí A, Jove M, Pamplona R, Barja
G.
Departamento de Fisiología Animal-II, Facultad de Ciencias Biológicas,
Complutense University, c/Jose Antonio Novais-2, Madrid 28040, Spain.
Dietary restriction (DR) lowers mitochondrial reactive oxygen species
(ROS) generation and oxidative damage and increases maximum longevity
in rodents. Protein restriction (PR) or methionine restriction (MetR),
but not lipid
[/quote]
would you please test the effect of SATURATED versus POLYUNSATURATED
fat and not just the corn/soybean oil thing called "lipid"?
[quote]or carbohydrate restriction, also cause those kinds of
changes.
[/quote]
We have shown just recently here that AGE (come supposedly from
carbohydrates) restriction DO have an effect ...
[quote]However, previous experiments of MetR were performed only at
80% MetR, and substituting dietary methionine with glutamate in the
diet. In order to clarify if MetR can be responsible for the lowered
ROS production and oxidative stress induced by standard (40%) DR,
Wistar rats were subjected to 40% or 80% MetR without changing other
dietary components. It was found that both 40% and 80% MetR decrease
mitochondrial ROS generation and percent free radical leak in rat
liver mitochondria, similarly to what has been previously observed in
40% PR and 40% DR. The concentration of complexes I and III, apoptosis
inducing factor, oxidative damage to mitochondrial DNA, five different
markers of protein oxidation, glycoxidation or lipoxidation and fatty
acid unsaturation were also lowered. The results show that 40%
isocaloric MetR is enough to decrease ROS production and oxidative
stress in rat liver. This suggests that the lowered intake of
methionine is responsible for the decrease in oxidative stress
observed in DR.
PMID: 18283555 [PubMed - in process]
[/quote]
The oxidative damage can be decreased either
1) at its origin within the ETC by this MetR or the adaptation to cold
climates (uncoupling)
2) on the way by stopping it with antioxidants or restricting the
catalysts such as iron
3) by changing to more resistant building blocks, i.e. substituting
Omega-3/6 PUFAs for Omega-9 PUFAs within the membranes
Taka |
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